By A. Krishna Mallia

This e-book is a pragmatic advisor to the practise and use of immobilized affinity ligands for purification, catalysis, and research. specific emphasis is given to immunochemical suggestions together with antibody isolation, instruction of antibody fragments utilizing immobilized enzymes, and immunoaffinity chromatography. The e-book offers easy-to-follow, well-tested protocols to permit the uninitiated to exploit those suggestions to the utmost virtue with minimal trouble. moreover, it exhibits researchers the right way to get monetary savings through making their very own optimized affinity supports.

Key Features
* Matrix activation
* Ligand immobilization
* Binding and elution of objective molecules
* Enzyme catalysis on reliable supports
* Analytical affinity chromatography
* Isolation/purification of antibodies
* guidance of antibody fragments
* Immunoaffinity chromatography
* Immobilization of nucleic acids
* Use of immobilized ligands for removing of hint contaminants
Practical suggestion on choosing:
* Matrices
* Spacers
* tools of activation and coupling
Background details and insights on:
* Affinity interactions
* the convenience and gear of affinity chromatography
* Attaching molecules to insoluble supports
* Matrices at the moment in use
* Over 20 tools of activation
* Spacers
* wide References

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